Semi-targeted method identifying up to 201 metabolites using UPLC-QTRAP MS/MS with specific MRMs and using several internal standards. Metabolites captured include amino acids, amine derivatives, one carbon metabolism compounds, neurotransmitters, glutathione, water-soluble vitamins, purines/pyrimidines and their nucleotides, and acylcarnitines. Although some of these same metabolites may also be detected in our Primary Metabolomics assay, this method detects the compounds described above with greater sensitivity (at lower concentrations). All metabolites have been verified and optimized using pure standards.
A total of 10 surrogate internal standards (stable isotope and deuterated) and an injection internal standard are utilized, as well as UTAK plasma for intra- and inter-batch variability assessment, in addition to replicate analysis of study samples.
Metabolite list is included below: